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  • Title: [Experimental study on the effect of Yiqi Wenyang Decoction on nasal mucosa infiltration of NK cells in mice with allergic rhinitis].
    Author: Chen XQ, Ma Q, Zhou LY, Ma HA, Wu JY, Zhao JJ, Yan DN.
    Journal: Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi; 2017 Dec 07; 52(12):921-926. PubMed ID: 29262451.
    Abstract:
    Objective: To observe the effect of Yiqi Wenyang Decoction on the infiltration and activation of NK cells in nasal mucosa of mouse model with allergic rhinitis (AR), and to explore the potential mechanism for effective intervention of AR with Yiqi Wenyang Decoction. Methods: Fourty-eight mice were randomly divided into blank group, model group, low, medium and high dose of Yiqi Wenyang Decoction group and Cetirizine group, with 8 rats in each group. After modeling of AR, the model group was filled with 0.9% sodium chloride solution. Yiqi Wenyang Decoction groups of each dose were given different concentrations of Yiqi Wenyang Decoction water extract, while the Cetirizine group was given aqueous solution of Cetirizine. The behavior, morphological changes of nasal mucosa and infiltration of NK cells in nasal mucosa were observed. The levels of IL-4 and INF-γ in nasal lavage fluid were measured. Besides, the drug safety was observed by acute toxicity test. Results: In the respect of behavioral scoring, middle and high dose of Yiqi Wenyang Decoction group were superior to the model group (number of sneezing: q value was 7.189, 8.748, respectively; number of scratching nose: q value was 12.074, 14.560, respectively; all P<0.05). In middle and high dose of Yiqi Wenyang Decoction group, the infiltration of NK cells and nasal lavage fluid IL-4 levels were lower than those in model group (IOD: q value was 10.073, 12.322, respectively; IOD/Area: q value was 10.954, 14.073, respectively; IL-4: q value was 4.705, 6.801, respectively; all P<0.05). There was no significant difference in nasal lavage fluid of INF-γ among each group (Fv=1.166, P>0.05). In acute toxicity test, no obvious poisoning symptoms and death occurred in mice. Conclusion: Yiqi Wenyang Decoction can control the nasal symptom, reduce the local NK cell infiltration of nasal mucosa and inhibit the expression of the 2-type cytokines released by NK cells, which may be related with the potential mechanism of effective intervention of AR with Yiqi Wenyang Decoction. 目的： 观察益气温阳方对变应性鼻炎(AR)模型小鼠鼻黏膜NK细胞浸润、活化作用的影响，探讨益气温阳方有效干预AR的潜在机制。 方法： 将48只小鼠随机分为空白组、模型组、益气温阳方低、中、高剂量组及西替利嗪组6组，每组8只。建立小鼠AR模型后，模型组灌胃0.9%氯化钠溶液，益气温阳方各剂量组灌胃不同浓度的益气温阳方水提液，西替利嗪组灌胃西替利嗪水溶液，空白组灌胃PBS溶液，观察各组小鼠行为表现、鼻黏膜病理形态变化及鼻黏膜NK细胞浸润情况，测定鼻腔灌洗液白细胞介素4(IL－4)、γ干扰素(INF－γ)水平。另以益气温阳方灌服小鼠进行急性毒性实验，初步观察其药物安全性。组间比较采用单因素方差分析，以P<0.05为差异有统计学意义。 结果： 益气温阳方中、高剂量组小鼠的喷嚏数及搔鼻数显著低于模型组，差异有统计学意义(喷嚏数q值分别为7.189、8.748，搔鼻数q值分别为12.074、14.560，P值均<0.05)。益气温阳方中、高剂量组小鼠鼻黏膜的NK细胞浸润程度及鼻腔灌洗液IL－4水平均显著低于模型组，差异有统计学意义(IOD的q值分别为10.073、12.322，IOD/Area的q值分别为10.954、14.073，IL－4的q值分别为4.705、6.801，P值均<0.05)。各组鼻腔灌洗液INF－γ的水平差异无统计学意义(F＝1.166，P>0.05)。急性毒性实验过程中，小鼠未见明显中毒症状及死亡发生。 结论： 益气温阳方能抑制AR鼻部症状，减少鼻黏膜局部NK细胞浸润，抑制NK细胞等释放的2型细胞因子表达，其有效干预AR的潜在机制可能与此有关。.
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