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  • Title: Effect of pretreatments on isolation of bioactive polysaccharides from spent coffee grounds using subcritical water.
    Author: Getachew AT, Cho YJ, Chun BS.
    Journal: Int J Biol Macromol; 2018 Apr 01; 109():711-719. PubMed ID: 29294335.
    Abstract:
    In this study, we used a novel approach to recover polysaccharide from spent coffee ground (SCG) by combining pretreatments and subcritical water hydrolysis (SCWH). The independent variables which affect SCWH were optimized using response surface methodology. The highest yield of SCG polysaccharides (SCGPSs) (18.25 ± 0.21%) was obtained using ultrasonic pretreatment and SCWH conditions of temperature (178.85 °C), pressure (20 bar), and extraction time (5 min). The extracted SCGPSs showed high antioxidant activity as measured using ABTS+ and DPPH radical scavenging assay with IC50 values of 1.83 ± 0.03 and 2.66 ± 0.13 mg/ml respectively. SCGPSs also showed in vitro hypoglycemic activities. The structural and thermal characterization of the polysaccharide showed that the extracted polysaccharide has a typical carbohydrate features. The results of this study suggested that the extracted polysaccharide could have a potential application in food and related industries.
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