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Title: Incorporation of atmospheric oxygen into the carbonyl functionality of the protochlorophyllide isocyclic ring. Author: Walker CJ, Mansfield KE, Smith KM, Castelfranco PA. Journal: Biochem J; 1989 Jan 15; 257(2):599-602. PubMed ID: 2930469. Abstract: Detached cucumber (Cucumis sativus L. var. Beit Alpha) cotyledons incubated in darkness with 5-aminolaevulinic acid and either 16O2 air (control) or 18O2 in N2 accumulated protochlorophyllide. This was converted into methyl phaeoporphyrin alpha 5 and analysed by mass spectrometry. The molecular ion of the methyl phaeoporphyrin alpha 5 derived from the 18O2 incubation was 2 mass units greater than that of the control, establishing that the oxo oxygen atom of the isocyclic ring is derived from atmospheric oxygen.[Abstract] [Full Text] [Related] [New Search]