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  • Title: Covalent Probes for Carbohydrate-Active Enzymes: From Glycosidases to Glycosyltransferases.
    Author: Xu Y, Uddin N, Wagner GK.
    Journal: Methods Enzymol; 2018; 598():237-265. PubMed ID: 29306437.
    Abstract:
    Covalent probes for glycosidases and glycosyltransferases are of great interest as tool compounds for chemical biology. For glycosidases, a sizable number of such probes have been developed from covalent glycosidase inhibitors. We review selected recent examples and highlight different design strategies, including probes based on photoaffinity labels and mechanism-based inhibitors, as well as their applications in biology and for activity-based protein profiling. In contrast to glycosidases, only a limited number of covalent probes have been reported to date for glycosyltransferases. We describe a new class of covalent probes for the retaining α-1,4-galactosyltransferase LgtC from Neisseria meningitidis. On the basis of these probes, we have developed an operationally simple two-step protocol for the fluorescent labeling of recombinant LgtC both in purified form and in cell lysates. In principle, our approach is also applicable to other bacterial glycosyltransferases. Among other applications, our protocol may therefore be particularly useful for imaging of the differential expression of these enzymes in different bacterial species and strains.
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