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  • Title: Identification of the rate-limiting step in serine proteinases from the effect of temperature on steady-state kinetics.
    Author: Fitzpatrick PF.
    Journal: Biochim Biophys Acta; 1989 Apr 06; 995(2):201-3. PubMed ID: 2930798.
    Abstract:
    The effect of temperature on the steady-state kinetics of porcine pancreatic elastase can be used to determine whether acylation or deacylation is the rate-limiting step in catalysis. If acylation is rate-limiting, kcat and kcat/Km will show the same temperature dependence. If deacylation is rate-limiting, kcat will show a greater temperature dependence than kcat/Km. The temperature dependence of the steady-state kinetic parameters of t-Boc-Ala-Ala-Pro-Ala p-nitroanilide and N-acetyl-Ala-Ala-alpha-Aza-Ala p-nitrophenyl ester have been determined and are consistent with this prediction.
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