These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Inorganic phosphate amplifies the effects of AMP and fructose-2,6-bisphosphate on yeast phosphofructokinase.
    Author: Przybylski F, Nissler K, Schellenberger W, Hofmann E.
    Journal: Biomed Biochim Acta; 1985; 44(11-12):1559-65. PubMed ID: 2936338.
    Abstract:
    Inorganic phosphate is an important regulator of yeast phosphofructokinase activity. In the absence of AMP and fructose-2,6-bisphosphate the dependence of enzyme activity on the concentration of inorganic phosphate is sigmoidal. AMP and fructose-2,6-bisphosphate increase the affinity of phosphofructokinase to inorganic phosphate. At low fructose-6-phosphate concentrations inorganic phosphate amplifies the activating effect of AMP and fructose-2,6-bisphosphate. Yeast phosphofructokinase is more sensitive to ATP inhibition in the absence of inorganic phosphate than in its presence. While in the absence of inorganic phosphate a definite ATP inhibition prevails even at high levels of AMP or fructose-2,6-bisphosphate, the ATP inhibition can be relieved by the cooperation of inorganic phosphate and fructose-2,6-bisphosphate. These effects of inorganic phosphate provide an explanation for the stimulation of glycolysis under anaerobic conditions by inorganic phosphate at unchanged concentrations of AMP and fructose-2,6-bisphosphate (Lagunas and Gancedo, Eur. J. Biochem. 137, 479-483 (1983)).
    [Abstract] [Full Text] [Related] [New Search]