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  • Title: Quantification of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine and 1-methyl-4-phenylpyridinium ion in brain tissue by gas chromatography/mass spectrometry.
    Author: Shih MC, Markey SP.
    Journal: Biomed Environ Mass Spectrom; 1986 Feb; 13(2):85-9. PubMed ID: 2937483.
    Abstract:
    A sensitive gas chromatographic/mass spectrometric assay has been developed to quantify 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) and 1-methyl-4-phenylpyridinium ion (MPP+) in brain tissue. MPTP was derivatized with isobutyl chloroformate and then analyzed using gas chromatography/mass spectrometry. MPP+, a known metabolite of MPTP, was reduced with NaB2H4 to 2H2-MPTP before derivatization. Deuterated isotopomers of MPTP and MPP+ were used as internal standards. The intra-assay coefficients of variation in the determination of 57.3 ng MPTP and 371.8 ng MPP+ in 100 mg of mouse brain tissue were 3.0% and 5.6% (n = 7), respectively. The detection limit corresponds to about 2 ng per sample of authentic MPTP.
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