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  • Title: Nucleotide trapping at the ATPase site of myosin subfragment 1 by a new interthiol crosslinking.
    Author: Chaussepied P, Mornet D, Kassab R.
    Journal: Proc Natl Acad Sci U S A; 1986 Apr; 83(7):2037-41. PubMed ID: 2938184.
    Abstract:
    When myosin subfragment 1 derivatives in which the reactive sulfhydryl SH1 has been blocked react with N,N'-p-phenylenedimaleimide or 5,5'-dithiobis(2-nitrobenzoic acid), the reactive sulfhydryl group SH2 of the 20-kDa domain is crosslinked with a thiol of the 50-kDa domain of the heavy chain. The crosslink induces the stable trapping of a significant amount of Mg2+-nucleotide in the ATPase site.
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