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  • Title: Identification of the released form of atrial natriuretic factor by the perfused rat heart.
    Author: Thibault G, Garcia R, Gutkowska J, Lazure C, Seidah NG, Chrétien M, Genest J, Cantin M.
    Journal: Proc Soc Exp Biol Med; 1986 May; 182(1):137-41. PubMed ID: 2938186.
    Abstract:
    Atrial natriuretic factor (ANF), released by the isolated perfused rat heart, was extracted from the perfusates by C18 Sep-Pak cartridges and then isolated by immunoaffinity chromatography and by reverse phase HPLC. About 500 ng of immunoreactive material were so obtained and submitted to amino acid sequencing. The C-terminal Tyr was detected by radiolabelling. Identification of these residues indicated that the primary structure corresponds to ANF (Ser 99-Tyr 126) which is identical to the circulating form in the rat. These results indicate that the ANF released by the atria corresponds to a short peptide. Therefore, its maturation process may therefore take place either intracellularly or during secretion and implicates a tryptic-like cleavage after a single Arg residue in position 98.
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