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  • Title: [Mechanism of mTOR Pathway in K562 cell Apoptosis Induced by Homoharringtonine].
    Author: Ding YH, Wu JJ, Wang Q, Deng ZK, Li YF.
    Journal: Zhongguo Shi Yan Xue Ye Xue Za Zhi; 2018 Feb; 26(1):105-109. PubMed ID: 29397826.
    Abstract:
    OBJECTIVE: To investigate the effect of homoharringtonine (HHT) on proliferation and apoptosis of CML cell line K562 cells and to explore its possible mechanism through mTOR pathway. METHODS: K562 cells were cultured with different concentrations of HHT or in its combination with mTOR inhibitor rapamycin (RAPA) for 24 hours. The cell viability was analyzed by CCK-8 assay, the cell apoptosis was detected by flow cytometry, the expressions of BCL-6, Caspase-3 and mTOR signal pathway related proteins was assayed by Western blot, the expression of BCL-6 mRNA was determined by RT-PCR. RESULTS: The HHT inhibited proliferation and induced apoptosis of K562 cells in a concentration-dependent manner(r=0.970). With the increasing of HHT concentration, the expression level mTOR signal pathway related proteins increased(r=0.908), while the mRNA and protein expression levels of BCL-6 decreased(rmRNA=-0.961, rprotein =-0.981), as compared with the HHT alone, the combination of HHT with RAPA could down-regulate the expression of mTOR signal pathway related protein and caspase-3, and up-regulated expression of BCL-6. CONCLUSION: HHT induces apoptosis of K562 cells by inhibiting BCL-6 expression through mTOR signal pathway.
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