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  • Title: Suicide selection of murine T helper clones specific for variable regions of the influenza hemagglutinin molecule.
    Author: Thomas DB, Skehel JJ, Mills KH, Graham CM.
    Journal: Eur J Immunol; 1986 Jul; 16(7):789-93. PubMed ID: 2941307.
    Abstract:
    A negative selection procedure has been developed to obtain murine T helper clones specific for variable regions of the influenza A hemagglutinin. T cell lines, established from mice primed by intranasal infection with X31 (H3N2) virus, were cross-stimulated with natural variant viruses of known primary sequence (either A/TEXAS/1/77 or A/ENG878/69) and proliferating cells eliminated by treatment with the cell cycle-specific drug 5-bromodeoxyuridine. After two suicide cycles, T cell lines were subtype specific and failed to recognize the natural variants. Clones were established by limiting dilution and their specificity was determined against a panel of viruses. Extensive diversity was evident in the reactivity of clones from individual donors, and two major T cell recognition sites were defined in the globular head region of the hemagglutinin molecule.
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