These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: [Analysis of the gamma interferon receptor in mice].
    Author: Cofano F, Fassio A, Cavallo G, Landolfo S.
    Journal: G Batteriol Virol Immunol; 1985; 78(1-6):70-6. PubMed ID: 2943626.
    Abstract:
    Natural murine interferon-gamma (IFN-gamma) produced by the T lymphoma, L12R4, stimulated with phorbol myristic acetate was purified by anti-murine IFN-gamma immunoadsorbent and labeled with 125I to study its binding to receptors of mouse cells. By adding increasing concentrations of unlabeled natural murine IFN-gamma in competition binding assays we determined a KD of 8.2 X 10(-10) M for L1210 cells and a number of receptors of about 3000. Moreover protease, but not endoglycosidase treatment of target cells prevented the subsequent binding of the ligand. These studies demonstrate that natural murine IFN-gamma binds in a specific manner and with high affinity to receptors on murine cell lines.
    [Abstract] [Full Text] [Related] [New Search]