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  • Title: [Genotype analysis of Toxoplasma gondii isolated strains from congenital teras and HIV-Toxoplasma co-infected patient in Jiangsu Province].
    Author: Yong-Hua Z, Zhao-Feng H, Yi-Wu M, Xue-Dong W, Yong-Liang X, Jian-Ping T.
    Journal: Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi; 2017 Apr 25; 29(3):329-333. PubMed ID: 29469524.
    Abstract:
    OBJECTIVE: To identify the genotype of Toxoplasma gondii isolated strains from a congenital teras (KS strain) and an HIV-Toxoplasma co-infected patient in Jiangsu Province. METHODS: T. gondii DNA of tachyzoites of a isolate from a congenital teras (KS strain) and blood DNA of an HIV-Toxoplasma co-infected patient in Jiangsu Province were extracted, and 11 loci were identified for the genotype by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP). RESULTS: The complete bands were obtained from the congenital teras (KS strain) and HIV-Toxoplasma co-infected patient in Jiangsu Province, and identified as T. gondii gene type I. CONCLUSIONS: T. gondii gene type I may be the dominant genotype strain of T. gondii among the women who have the abnormal pregnant outcomes and HIV-Toxoplasma co-infected patients in Jiangsu Province. [摘要]目的 鉴定分离自江苏省先天性畸胎的弓形虫虫株 (KS株) 和HIV-弓形虫共感染患者刚地弓形虫的基因型。方法 抽提江苏省先天性畸胎弓形虫分离株 (KS株) 速殖子和1例HIV-弓形虫共感染患者的全血DNA, 选择11个基因位 点, 采用聚合酶链式反应-限制性片段长度多态性分析 (PCR-RFLP) 进行基因型鉴定。结果 自江苏省先天性畸胎分离 的弓形虫虫株和1例HIV-弓形虫共感染患者的全血DNA样本中均获得完整分型条带, 经鉴定均为弓形虫基因型Ⅰ型。结论 Ⅰ型刚地弓形虫可能是江苏地区引起妊娠异常结局妇女和HIV-弓形虫共感染患者的优势基因型虫株。.
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