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  • Title: Rhamnolipid from Pseudomonas aeruginosa inactivates mammalian tracheal ciliary axonemes.
    Author: Hastie AT, Hingley ST, Higgins ML, Kueppers F, Shryock T.
    Journal: Cell Motil Cytoskeleton; 1986; 6(5):502-9. PubMed ID: 2947698.
    Abstract:
    Isolated ciliary axonemes from pig trachea were exposed to increasing concentrations of purified Pseudomonas aeruginosa rhamnolipid. This is a defined ciliary system allowing observation of direct impairment of functional axonemes. Axonemal motility and ATPase activity were decreased in proportion to rhamnolipid concentrations. ATPase-associated proteins observed in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and dynein arms seen in ultra-structural cross sections progressively disappeared from axonemes with exposure to rhamnolipid. These four independent measures establish that the rhamnolipid removes the ATPase-containing outer dynein arms from the ciliary axoneme, thereby rendering the axoneme immotile.
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