These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Gingival Epithelial Cell Expression of Macrophage Inflammatory Protein-1α Induced by Interleukin-1β and Lipopolysaccharide.
    Author: Ryu OH, Choi SJ, Linares AMG, Song IS, Kim YJ, Jang KT, Hart TC.
    Journal: J Periodontol; 2007 Aug; 78(8):1627-1634. PubMed ID: 29539188.
    Abstract:
    BACKGROUND: Elevated levels of the macrophage inflammatory protein-1α (MIP-1α) are reported in inflammatory bone diseases including periodontitis. We evaluated the ability of interleukin-1β (IL-1β) and bacterial lipopolysaccharides (LPSs) to modulate MIP-1α expression in epithelial cells, fibroblasts, and polymorphonuclear leukocytes (PMNs). We also evaluated the effect of MIP-1α as an osteoclast activating factor. METHODS: Human gingival epithelial cells and fibroblasts were obtained by primary cell culture. PMNs were isolated from healthy controls. Human MG63 osteosarcoma cells were used as osteoblastic cells. After incubation of each cell type with IL-1β, Porphyromonas gingivalis LPS, and Actinobacillus actinomycetemcomitans LPS, MIP-1α mRNA and secreted protein levels were quantified by reverse transcription-polymerase chain reaction, enzyme-linked immunosorbent assay, and immunohistochemistry. The ability of recombinant MIP-1α to induce osteoclast formation was determined by tartrate resistant acid phosphatase assay. RESULTS: MIP-1α expression in PMNs and gingival epithelial cells was induced by IL-1β and LPS, but neither induced MIP-1α expression in gingival fibroblasts or osteoblastic cells. MIP-1α was highly expressed in the basal epithelial layer of inflamed gingiva but not in healthy gingiva. MIP-1α induced osteoclast formation at an optimal concentration of 0.05 to 2 ng/ml. CONCLUSIONS: MIP-1α expression by gingival epithelial cells may be important in initiating inflammation by facilitating accumulation and activation of leukocytes. The ability of MIP-1α to facilitate formation of multinuclear bone cells indicates a possible role in periodontitis-associated bone destruction. These findings indicate MIP-1α may play an important role in early and later stages of inflammatory-related periodontitis.
    [Abstract] [Full Text] [Related] [New Search]