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  • Title: Partial characterization of GTP-binding proteins in Neurospora.
    Author: Hasunuma K, Miyamoto-Shinohara Y, Furukawa K.
    Journal: Biochem Biophys Res Commun; 1987 Aug 14; 146(3):1178-83. PubMed ID: 2956953.
    Abstract:
    Six fractions of GTP-binding proteins separated by gel filtration of a mycelial extract containing membrane components of Neurospora crassa were partially characterized. [35S]GTP gamma S bound to GTP-binding protein was assayed by repeated treatments with a Norit solution and centrifugation. The binding of [35S]GTP gamma S to GTP-binding proteins was competitively prevented in the presence of 0.1 to 1 mM GTP but not in the presence of ATP. These GTP-binding proteins fractionated by the gel column had Km values of 20, 7, 4, 4, 80 and 2 nM. All six fractions of these GTP-binding proteins showed the capacity to be ADP-ribosylated by pertussis toxin.
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