These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Dose-dependent effects of frutalin on in vitro maturation and fertilization of pig oocytes.
    Author: Silva BR, Maside C, Vieira LA, Cadenas J, Alves BG, Ferreira ACA, Aguiar FLN, Silva ALC, Figueiredo JR, Silva JRV.
    Journal: Anim Reprod Sci; 2018 May; 192():216-222. PubMed ID: 29580752.
    Abstract:
    The aim of the present study was to evaluate the effect of frutalin (FTL) on in vitro maturation (IVM), and fertilization (IVF) of pig oocytes. In the Experiment 1, cumulus-oocyte complexes (COCs) were submitted to IVM in maturation medium alone or supplemented with different FTL concentration (0.6, 6 and 60 μg/mL), or 0.3 μg/mL doxorubicin (DXR). After IVM, some oocytes were evaluated for chromatin configuration, and the remaining oocytes were submitted to in vitro fertilization. In Experiment 2, matured oocytes were fertilized in IVF medium alone (control) or in presence of different FTL concentration (0.6, 6 and 60 μg/mL), or 0.3 μg/mL DXR. After 18 h post fertilization, the endpoints penetration rate, monospermy, spermatozoa per oocyte, and the IVF efficiency were evaluated in both experiments. In Experiment 1, 6 and 60 μg/mL FTL, as well as DXR increased (P < 0.05) the rate of oocytes with abnormal chromatin configuration when compared to oocyte matured in control medium alone or supplemented with 0.6 μg/mL FTL. The percentage of meiotic resumption in oocytes cultured with 60 μg/mL FTL or DXR was less (P < 0.05) than in the other treatments. Moreover, oocytes matured with 6 or 60 μg/mL FTL and DXR had a lesser IVM efficiency when compared to those matured with 0.6 μg/mL FTL or in control medium. Additionally, there was a greater (P < 0.05) with culture in a medium containing 6 μg/mL FTL for the rate of partenogenetically activated oocytes when compared with the other treatments. Culturing of COCs during IVM in a medium containing 6 or 60 FTL resulted in a lesser (P < 0.05) sperm penetration and spermatozoa/oocyte rates when compared to other treatments, and IVF efficiency was less (P < 0.05) than that in control medium alone or with a medium containing 0.6 μg/mL FTL. In Experiment 2, culturing in a medium containing 0.6 μg/mL FTL resulted in greater (P < 0.05) monospermy and IVF efficiency rates when compared to culturing in the control medium. In addition, culturing in a medium with 6 and 60 μg/mL FTL resulted in a lesser (P < 0.05) spermatozoa penetration, sperm/oocyte rates and IVF efficiency, although there were greater (P < 0.05) monospermy rates. In conclusion, culturing in a medium containing 0.6 μg/mL FTL resulted in lesser spermatozoa penetration rates and number of spermatozoa/oocyte increasing the IVF efficiency without harmful effects. Use of a greater concentration of FTL in the medium has toxic effects during oocyte maturation and results in a reduced IVF efficiency.
    [Abstract] [Full Text] [Related] [New Search]