These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Growth kinetics of the G2-phase of Ehrlich ascites tumor cells, separated from anaerobically treated asynchronous cultures.
    Author: Kroll C, Kroll W, Schneider F.
    Journal: Z Naturforsch C J Biosci; 1987; 42(7-8):991-8. PubMed ID: 2961157.
    Abstract:
    Cell cycle progression of G2 fractions (75-80% G2 (4C) cells) from 8 h anaerobically cultured asynchronous hyperdiploid Ehrlich ascites tumor cells strain Karzel, separated by centrifugal elutriation, was studied after reaeration by flow cytometric methods, including the BrdU-H33258 technique and dual parameter measurements. Analyses of the growth kinetics demonstrated that one fraction of the cell population proceeds through a normal cell cycle (2C----4C) with a generation time of about 20 h. Another portion entered a new cycle (4C----8C) to form cells with a DNA content up to 8C; mono-, bi- and polynucleate cells could be detected. After 15 h aerobic recultivation of the anaerobically cultured G2 cells, a fraction containing 80-85% with a DNA content of greater than 4C was separated. On recultivation, these cells pass a 4C----8C division cycle with a generation time of about 10 h, and a G1 period of less than 4 h.
    [Abstract] [Full Text] [Related] [New Search]