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  • Title: Natural saliva as an adjuvant in a secondary caries model based on Streptococcus mutans.
    Author: Hetrodt F, Lausch J, Meyer-Lueckel H, Apel C, Conrads G.
    Journal: Arch Oral Biol; 2018 Jun; 90():138-143. PubMed ID: 29614462.
    Abstract:
    OBJECTIVE: Two factors for secondary caries formation were evaluated using an artificial biofilm model, saliva as additive in culture medium and bonding procedures of composite materials for artificial gap creation. DESIGN: Standardized cavities were prepared in bovine tooth samples (n = 44), treated with two different bonding pretreatments, restored and after artificial ageing incubated with Streptococcus mutans in a Mueller-Hinton-Broth-Sugar medium with or without human saliva for seven days. Secondary caries formation was analyzed using confocal laser scanning microscopy and transversal microradiography. RESULTS: Lesions were significantly pronounced in groups using saliva, but were not influenced by the bonding pretreatments. CONCLUSIONS: The results indicate that the addition of saliva, but not the type of bonding procedure influences the outcome in the present biofilm-based secondary caries model.
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