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  • Title: [The study on human placental DHA-S transport mechanism (using placental microvillous membrane vesicles)].
    Author: Iioka H, Moriyama I, Akazaki M, Oku M, Itoh K, Hino K, Okamura Y, Itani Y, Katoh Y, Ichijo M.
    Journal: Nihon Sanka Fujinka Gakkai Zasshi; 1987 Oct; 39(10):1756-60. PubMed ID: 2963081.
    Abstract:
    To investigate the placental DHA-S (dehydroepiandrosterone sulfate) transport mechanism, the uptake of DHA-S into microvillous membrane vesicles prepared from human term placenta was studied using the rapid filtration technique. 1. The uptake of DHA-S into microvillous membrane vesicles was not dependent on both Na+ electrochemical gradient and membrane potential difference. 2. The uptake of DHA-S into microvillous membrane vesicles was dependent on temperature. The initial uptake rate of DHA-S at 37 degrees C was three times as great as at 4 degrees C. 3. The initial rate of DHA-S transport exhibited saturation kinetics with respect to the DHA-S concentration; an apparent Km of 0.067 mM and Vmax of 1.01 nmol/mg protein/20 sec were calculated. 4. The uptake of DHA-S into microvillous membrane vesicles was inhibited by DHA, but not by estriol. These results indicated that placental DHA-S transport was carrier mediated and a passive one.
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