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  • Title: Specific receptors for thromboxane A2 in cultured vascular smooth muscle cells of rat aorta.
    Author: Hanasaki K, Nakano K, Kasai H, Arita H, Ohtani K, Doteuchi M.
    Journal: Biochem Biophys Res Commun; 1988 Feb 15; 150(3):1170-5. PubMed ID: 2963633.
    Abstract:
    The specific binding site for thromboxane A2 (TXA2) was studied in cultured vascular smooth muscle cells (VSMC) of the rat aorta. [3H]SQ29,548, a potent and selective TXA2 receptor antagonist, displayed high-affinity and specificity, as well as saturable and displaceable binding to rat VSMC in culture. Scatchard analysis of equilibrium binding at 24 degrees C revealed a single class of binding sites with a Kd of 1.7 nM and a Bmax of 8.0 fmol/10(6) cells. A series of TXA2 receptor antagonists completely suppressed [3H]SQ29,548 binding to rat VSMC, and the rank order of their inhibitory potencies (Ki) correlated well with the potencies for suppression of the U46619-induced contraction of rat thoracic aorta. These results suggest that specific binding sites for [3H]SQ29,548 represent the TXA2 receptor in rat VSMC.
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