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  • Title: The J gene of phi X174: isolation and characterization of a J gene mutant.
    Author: Hamatake RK, Buckley KJ, Hayashi M.
    Journal: Mol Gen Genet; 1988 Jan; 211(1):72-7. PubMed ID: 2963945.
    Abstract:
    The J gene protein of bacteriophage phi X174 is a component of the mature phage. The previous lack of J gene mutants has prevented an in vivo analysis of J protein functions. A phi X174 mutant was constructed by inserting an 11 nucleotide sequence into the J gene. This mutant, designated insJ, was viable only in the presence of a wild-type J gene carried on a plasmid that could provide J protein. An analysis of DNA synthesis during insJ mutant infection under non-permissive conditions confirmed that the J protein is not required for viral DNA synthesis.
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