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  • Title: In Vitro Fertilization in Mice.
    Author: Takeo T, Nakagata N.
    Journal: Cold Spring Harb Protoc; 2018 Jun 01; 2018(6):. PubMed ID: 29669849.
    Abstract:
    The success of in vitro fertilization is strongly influenced by genetic background, handling of sperm and oocytes, and culture conditions. This protocol promotes enhanced rates of fertilization by using preincubation medium for sperm containing methyl-β-cyclodextrin (MBCD) and fertilization medium with high calcium and reduced glutathione (GSH).
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