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Title: Heat-shock-induced cathepsin B activity during IVF and culture compromises the developmental competence of bovine embryos. Author: Yamanaka KI, Khatun H, Egashira J, Balboula AZ, Tatemoto H, Sakatani M, Takenouchi N, Wada Y, Takahashi M. Journal: Theriogenology; 2018 Jul 01; 114():293-300. PubMed ID: 29677632. Abstract: Heat stress can cause significant reproductive dysfunction in mammals and previous studies report that expression and activity of cathepsin B (CTSB), a lysosomal cysteine protease, is negatively correlated with the developmental competence of bovine oocytes and embryos. However, the relationship between heat shock (HS) and CTSB remains largely unknown. Here, we investigated the effects of HS during IVF and early embryonic stages of IVC on CTSB activity and developmental competence in bovine embryos. HS (40 °C for 6 h during IVF and 20 h during IVC) caused a significant increase in CTSB activity irrespective of the developmental stage or duration of HS. The developmental rate to the blastocyst stage was also significantly decreased by HS. Additionally, HS during IVC significantly increased the number of apoptotic cells in blastocysts. Notably, these HS-induced changes in blastocyst development and quality were significantly improved by inhibition of CTSB activity, indicating a key role for CTSB. These results showed that CTSB activity plays an essential role in HS-induced dysfunction in bovine embryo development, and that inhibition of this activity could enhance the developmental competence of heat-shocked embryos.[Abstract] [Full Text] [Related] [New Search]