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  • Title: The ATP-driven primary Na+ pump in subcellular vesicles of Vibrio alginolyticus.
    Author: Dibrov PA, Skulachev VP, Sokolov MV, Verkhovskaya ML.
    Journal: FEBS Lett; 1988 Jun 20; 233(2):355-8. PubMed ID: 2968282.
    Abstract:
    Subcellular vesicles of Vibrio alginolyticus hydrolyze ATP and accumulate Na+ in an ATP-dependent fashion. The Na+ uptake is (i) strongly stimulated by delta psi-discharging agents, i.e., the protonophorous uncoupler CCCP or valinomycin + K+ and (ii) arrested by DCCD at a concentration strongly inhibiting ATP hydrolysis. Lower concentrations of DCCD stimulate the Na+ accumulation supported by ATP hydrolysis as well as by NADH oxidation. It is concluded that there is an electrogenic DCCD-sensitive Na+-ATPase in the cytoplasmic membrane of V. alginolyticus.
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