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  • Title: LncRNA RGMB-AS1 is activated by E2F1 and promotes cell proliferation and invasion in papillary thyroid carcinoma.
    Author: Zhang Z, Li SY, Zhang LB.
    Journal: Eur Rev Med Pharmacol Sci; 2018 Apr; 22(7):1979-1986. PubMed ID: 29687852.
    Abstract:
    OBJECTIVE: To detect the expression of long non-coding ribonucleic acid (lncRNA) RGMB-AS1 in papillary thyroid carcinoma (PTC) and to investigate its influences on PTC cell biological behaviors and its relevant molecular mechanisms. PATIENTS AND METHODS: The expression levels of lncRNA RGMB-AS1 in human PTC tissues, corresponding normal tissues, normal thyroid epithelial cells Nthyori3-1, human PTC cells TPC-1, BCPAP and K1 were detected by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Si-RGMB-AS1 and control sequence were transfected into TPC-1 and K1 cells. Changes in cell proliferation were detected via cell counting kit 8 (CCK8) assay, and changes in cell migration and invasion capacities were detected by transwell assay. Bioinformatics software was used to predict that the transcription of lncRNA RGMB-AS1 was regulated by the transcription factor E2F1, and changes in lncRNA RGMB-AS1 expression were detected by qRT-PCR after interference in E2F1 expression. Moreover, changes in cell biological functions were detected by CCK8 and transwell assays. Finally, chromatin immunoprecipitation (CHIP) assay was used to detect whether E2F1 bound to lncRNA RGMB-AS1 promoter region. RESULTS: Results of qRT-PCR showed that the lncRNA RGMB-AS1 expression was up-regulated in 38 out of 48 cases of PTC tissues, and it was also up-regulated in PTC cells. Results of CCK8 assay showed that the proliferation capacity of PTC cells was decreased after interference in the expression of lncRNA RGMB-AS1, and results of transwell assay revealed that cell invasion and migration capacities were inhibited. qRT-PCR showed that after interference in E2F1, the expression of lncRNA RGMB-AS1 was down-regulated. Besides, CCK8 and transwell assays showed that proliferation, migration, and invasion capacities of PTC cells were decreased after interference in E2F1. Results of CHIP assay showed that E2F1 bound to lncRNA RGMB-AS1 promoter region. CONCLUSIONS: E2F1 promotes the transcription of lncRNA RGMB-AS1 in PTC. Highly-expressed lncRNA RGMB-AS1 promotes the proliferation, invasion, and migration of PTC.
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