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  • Title: Isolation and Structural Characterization of Antioxidant Peptides from Degreased Apricot Seed Kernels.
    Author: Zhang H, Xue J, Zhao H, Zhao X, Xue H, Sun Y, Xue W.
    Journal: J AOAC Int; 2018 Sep 01; 101(5):1661-1663. PubMed ID: 29724259.
    Abstract:
    BACKGROUND: The composition and sequence of amino acids have a prominent influence on the antioxidant activities of peptides. OBJECTIVE: A series of isolation and purification experiments was conducted to explore the amino acid sequence of antioxidant peptides, which led to its antioxidation causes. METHODS: The degreased apricot seed kernels were hydrolyzed by compound proteases of alkaline protease and flavor protease (3:2, u/u) to prepare apricot seed kernel hydrolysates (ASKH). ASKH were separated into ASKH-A and ASKH-B by dialysis bag. ASKH-B (MW < 3.5 kDa) was further separated into fractions by Sephadex G-25 and G-15 gel-filtration chromatography. Reversed-phase HPLC (RP-HPLC) was performed to separate fraction B4b into two antioxidant peptides (peptide B4b-4 and B4b-6). RESULTS: The amino acid sequences were Val-Leu-Tyr-Ile-Trp and Ser-Val-Pro-Tyr-Glu, respectively. CONCLUSIONS: The results suggested that ASKH antioxidant peptides may have potential utility as healthy ingredients and as food preservatives due to their antioxidant activity. HIGHLIGHTS: Materials with regional characteristics were selected to explore, and hydrolysates were identified by RP-HPLC and matrix-assisted laser desorption ionization-time-of-flight-MS to obtain amino acid sequences.
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