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  • Title: Improvement of Trehalose Production by Immobilized Trehalose Synthase from Thermus thermophilus HB27.
    Author: Sun J, Wang S, Li W, Li R, Chen S, Ri HI, Kim TM, Kang MS, Sun L, Sun X, Yuan Q.
    Journal: Molecules; 2018 May 04; 23(5):. PubMed ID: 29734676.
    Abstract:
    Trehalose is a non-reducing disaccharide with a wide range of applications in the fields of food, cosmetics, and pharmaceuticals. In this study, trehalose synthase derived from Thermus thermophilus HB27 (TtTreS) was immobilized on silicalite-1-based material for trehalose production. The activity and the stability of TtTreS against pH and temperature were significantly improved by immobilization. Enzyme immobilization also led to a lower concentration of byproduct glucose, which reduces byproduct inhibition of TtTreS. The immobilized TtTreS still retained 81% of its initial trehalose yield after 22 cycles of enzymatic reactions. The immobilized TtTreS exhibited high operational stability and remarkable reusability, indicating that it is promising for industrial applications.
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