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  • Title: Directional K+ channel insertion in a single phospholipid bilayer: Neutron reflectometry and electrophysiology in the joint exploration of a model membrane functional platform.
    Author: Rondelli V, Del Favero E, Brocca P, Fragneto G, Trapp M, Mauri L, Ciampa MG, Romani G, Braun CJ, Winterstein L, Schroeder I, Thiel G, Moroni A, Cantu' L.
    Journal: Biochim Biophys Acta Gen Subj; 2018 Aug; 1862(8):1742-1750. PubMed ID: 29753114.
    Abstract:
    We investigated the insertion of small potassium (K+) channel proteins (KcvMA-1D and KcvNTS) into model membranes and the lipid-protein structural interference, combining neutron reflectometry and electrophysiology. Neutron reflectometry experiments showed how the transverse structure and mechanical properties of the bilayer were modified, upon insertion of the proteins in single model-membranes, either supported on solid substrate or floating. Parallel electrophysiology experiments were performed on the same channels reconstituted in free-standing planar lipid bilayers, of both typical composition and matched to the neutron reflectometry experiment, assessing their electrical features. Functional and structural results converge in detecting that the proteins, conical in shape, insert with a directionality, cytosolic side first. Our work addresses the powerful combination of the two experimental approaches. We show here that membrane structure spectroscopy and ion channel electrophysiology can become synergistic tools in the analysis of structural-functional properties of biomimetic complex environment.
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