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  • Title: Storage of spheroplasts at -70 degrees C for transfection with phi X174 RF DNA.
    Author: Malling HV, Fater MC, Burkhart JG, Hardies SC, Hutchison CA, Edgell MH.
    Journal: Gene Anal Tech; 1987; 4(2):23-6. PubMed ID: 2976728.
    Abstract:
    A method was developed for preparation of spheroplasts used for transfection with phi X174 RF DNA. This method had a high-level competence and retained the competence for up to one year of storage in 7% DMSO at -70 degrees C.
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