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  • Title: Sensitive and label-free electrochemical lead ion biosensor based on a DNAzyme triggered G-quadruplex/hemin conformation.
    Author: Wang L, Wen Y, Li L, Yang X, Jia N, Li W, Meng J, Duan M, Sun X, Liu G.
    Journal: Biosens Bioelectron; 2018 Sep 15; 115():91-96. PubMed ID: 29803866.
    Abstract:
    Lead ion (Pb2+) is a common environmental contaminant, which causes serious bioaccumulation and toxicity in human body. In this work, we developed a novel Pb2+ electrochemical biosensor using the specific DNAzyme on a DNA tetrahedron probe, in the presence of Pb2+, the substrate strand was cleaved into two parts and released a "G-rich" oligo which subsequently formed a G-quadruplex/hemin complex, generating a detectable catalysis current signal with the assistant of H2O2. The 3-D DNA tetrahedron regulated the density and orientation of the probe and thus improved the DNAzyme reaction, and facilitated the complex DNA conformational change in the confined space of the interface on the electrode surface, Finally, the LOD of our biosensor was proved to be 0.008 nM (3σ), which is 9000 times lower than the safety limit of EPA (15 μg/L or 72 nM), and 6000 times lower than IARC (10 μg/L or 48.26 nM), and more importantly, the specificity and reproducibility of the proposed biosensor was well demonstrated.
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