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  • Title: Proton-coupled transport of glycylglycine in rabbit renal brush-border membrane vesicles.
    Author: Takuwa N, Shimada T, Matsumoto H, Hoshi T.
    Journal: Biochim Biophys Acta; 1985 Mar 28; 814(1):186-90. PubMed ID: 2983762.
    Abstract:
    Transport of glycylglycine into rabbit renal brush-border membrane vesicles was found to be Na+-independent, H+ gradient-dependent and electrogenic. Marked overshoot uptake of the dipeptide was observed when an inward-directed proton gradient and inside-negative potential difference were imposed simultaneously across the vesicular membranes. Saturable depolarization of vesicular membranes could be demonstrated with glycylglycine by use of a fluorescent cyanine dye, di-S-C3(5). The results indicate that glycylglycine is contransported with H+ across the membranes.
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