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  • Title: Insulin release independent of a rise in cytosolic free Ca2+ by forskolin and phorbol ester.
    Author: Tamagawa T, Niki H, Niki A.
    Journal: FEBS Lett; 1985 Apr 22; 183(2):430-2. PubMed ID: 2985438.
    Abstract:
    The role of cytosolic free Ca2+ in insulin release was evaluated using isolated rat pancreatic islets permeabilized with digitonin and incubated in Ca-EGTA buffers to fix free Ca2+ concentration at arbitrary levels. Ca2+ induced insulin release in a concentration-dependent manner with the threshold being between 0.1 and 1 microM. The hormone release was increased by forskolin and 12-O-tetradecanoyl phorbol-13-acetate (TPA), a potent activator of adenylate cyclase and that of protein kinase C, respectively. The findings suggest that activation of both protein kinase A and protein kinase C modulate insulin release without a concomitant increase in cytosolic free Ca2+.
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