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  • Title: A Label-Free Aptasensor for Ochratoxin a Detection Based on the Structure Switch of Aptamer.
    Author: Liu F, Ding A, Zheng J, Chen J, Wang B.
    Journal: Sensors (Basel); 2018 Jun 01; 18(6):. PubMed ID: 29857594.
    Abstract:
    A label-free sensing platform is developed based on switching the structure of aptamer for highly sensitive and selective fluorescence detection of ochratoxin A (OTA). OTA induces the structure of aptamer, transforms into G-quadruplex and produces strong fluorescence in the presence of zinc(II)-protoporphyrin IX probe due to the specific bind to G-quadruplex. The simple method exhibits high sensitivity towards OTA with a detection limit of 0.03 nM and excellent selectivity over other mycotoxins. In addition, the successful detection of OTA in real samples represents a promising application in food safety.
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