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  • Title: Structure-activity relationship of porphyrin-induced photoinactivation with membrane function in bacteria and erythrocytes.
    Author: Kato H, Komagoe K, Inoue T, Masuda K, Katsu T.
    Journal: Photochem Photobiol Sci; 2018 Jul 11; 17(7):954-963. PubMed ID: 29892767.
    Abstract:
    We analyzed the structure-activity relationship of porphyrins with the photoinactivation of membrane function in bacteria and erythrocytes. The porphyrins tested were protoporphyrin (PP), mesoporphyrin (MP), deuteroporphyrin (DP), hematoporphyrin (HP), coproporphyrin (CP) and uroporphyrin (UP), along with hematoporphyrin derivative (HPD) and photofrin (PF). These porphyrins dissipated membrane potential of Staphylococcus aureus cells depending on the degrees of respiratory inhibition and K+ leakage. The dysfunction of bacterial membrane was caused within minutes and in the order of PP ∼ MP > DP > HPD ≫ HP > PF > CP ∼ UP. For bovine erythrocytes, these porphyrins induced leakage of K+ and inhibition of the enzyme acetylcholinesterase, which is located on the outer layer of the erythrocyte membrane, in the same order as that observed in bacteria. At high concentrations of PP, MP, DP and HPD, hemolysis (the lysis of erythrocytes with liberation of hemoglobin) was also induced. We found that the degree of photoinactivation of membrane function was closely associated with porphyrin-induced morphological changes in bovine erythrocytes, forming a crenated form from the normal discoid, which is the index of the amount of porphyrins in the outer layer of the cytoplasmic membrane. Furthermore, the degree of morphological changes was related with the octanol/water partition coefficients of porphyrins. These results strongly supported that porphyrins located in the outer layer of cytoplasmic membrane inactivated the cell membrane function by photo-irradiation, and the strength of photoinactivation by porphyrins depended on their affinity to the cell membrane.
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