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  • Title: [Carboxylated hapten coated directly coated ELISA for the detection of atrazine residue in water].
    Author: Sun W, Huang G, Sai N, Xu B.
    Journal: Wei Sheng Yan Jiu; 2017 Jan; 46(1):109-119. PubMed ID: 29903162.
    Abstract:
    OBJECTIVE: To develop the carboxylated hapten coated enzyme-linked immunosorbent assay( ELISA) for the detection of atrazine in drinking water. METHODS: Polystyrene surface was modified by 3-aminopropyltriethoxysilane( APTES) to produce amino groups for the directly immobilization of carboxylated atrazine on the surface of microtiter plates. RESULTS: The carboxylated hapten coated directly coated ELISA showed higher sensitivity( 0. 68 ng / m L) and higher specificity. In real sample analysis, the recoveries were ranged from 94. 0% to 112. 0%, and the relative standard deviation was 2. 72%- 3. 53%. CONCLUSION: The method is simple, reliable, and can be used to detect atrazine in drinking water.
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