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  • Title: Electric field mediated transformation: isolation and characterization of a TK+ subclone.
    Author: Zerbib D, Amalric F, Teissié J.
    Journal: Biochem Biophys Res Commun; 1985 Jun 28; 129(3):611-8. PubMed ID: 2990461.
    Abstract:
    Transformation of mammalian TK- cells by a plasmid carrying the TK gene from Herpes virus simplex 1 (pAGO) was mediated by electroporation. The cells were treated either in suspension or growing in monolayers directly in the petri dish. The yield of transformation was between 8.10(-5) and 2.10(-4) per microgram DNA depending on the experimental conditions. The structure of the integrated DNA was investigated proving the occurrence of a duplication process that affected preferentially the pBR322 part of the pAGO DNA (60 copies per cell). The TK gene that gave the TK+ phenotype to the selected clone was present in less than 6 copies.
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