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  • Title: Glucocorticoid-stimulated biosynthesis of low density lipoprotein receptor in cultured fibroblasts.
    Author: Filipovic I, Buddecke E.
    Journal: J Clin Chem Clin Biochem; 1985 Jun; 23(6):331-6. PubMed ID: 2991417.
    Abstract:
    Preincubation of human skin fibroblasts in the presence of 10(-6)-10(-5) mol/l glucocorticoids (dexamethasone) causes a concentration and time-dependent increase of receptor-mediated internalisation of [125I]LDL. This increase is due to a glucocorticoid-specific stimulation by 40-50% of LDL receptor synthesis as demonstrated by an increased incorporation of [35S]methionine into immune precipitated receptor protein. In contrast the rate of synthesis of total cell protein and of lysosomal cathepsin D is not significantly influenced by dexamethasone. The increased LDL receptor synthesis is accompanied by an enhanced synthesis of cholesterol from [2-3H]mevalonolactone and [1-14C]acetate. The glucocorticoid-induced enhancement of LDL receptor and cholesterol synthesis is abolished by preincubation of the cells with dexamethasone in combination with 25-hydroxycholesterol.
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