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  • Title: Comparison of Tris egg yolk-based, Triladyl® and Optixell® extender on post-thaw quality, Kinematics and in vivo fertility of Nili Ravi Buffalo (Bubalus bubalis) bull spermatozoa.
    Author: Naz S, Umair M, Iqbal S.
    Journal: Andrologia; 2018 Oct; 50(8):e13063. PubMed ID: 29920732.
    Abstract:
    Our objectives were to ascertain the comparison of Tris egg yolk-based, Triladyl® and Optixell® extender on postthaw quality, CASA parameters and in vivo fertility of Nili Ravi buffalo (Bubalus bubalis) bull spermatozoa. Semen samples (n = 35) from five bulls were diluted in Tris egg yolk-based, Triladyl® , Optixell® extender and frozen in 0.50 ml French straws. Postthaw sperm CASA motility (%) was higher (p < 0.05) in Optixell extender as compared to Triladyl and Tris egg yolk-based extender. Although sperm progressive motility (%), morphology (%), average path velocity (μm/s), straight line velocity (μm/s), curvilinear velocity (μm/s), amplitude of lateral head displacement (μm), beat cross-frequency (Hz), straightness (%), length of curvilinear path (μm), length of average path (μm), intact plasma and acrosome membrane (%), and DNA integrity (%) were higher (p < 0.05) in spermatozoa cryopreserved in Optixell® extender as compared to Tris egg yolk-based and Triladyl® extender. The fertility rates (68.18%, 45.45%, 55.4%) were higher (p < 0.05) in buffaloes inseminated with semen doses frozen in Optixell extender than the Tris egg yolk-based and Triladyl® extender respectively. It is concluded that Optixell® extender improves postthaw semen quality and fertility in Nili Ravi buffaloes.
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