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  • Title: "Differential displacement"--a simple method to study receptors for 1,25-dihydroxyvitamin D3 in the presence of contaminating serum vitamin D binding protein.
    Author: Levy FO, Eikvar L, Frøysa A, Hansson V.
    Journal: Biochem Biophys Res Commun; 1985 Aug 15; 130(3):1020-6. PubMed ID: 2992493.
    Abstract:
    This report describes a simple procedure to quantitate receptors for 1,25-dihydroxyvitamin D3 in the presence of contaminating serum vitamin D binding protein. The method ("differential displacement") takes advantage of the greatly different rates of dissociation of 1,25-dihydroxyvitamin D3 from the serum vitamin D binding protein (t 1/2 less than 5 min) and from the receptor (t 1/2 greater than 120 h) at 0 degrees C. The principle of "differential displacement" can be used for other steroid receptors as well, and in combination with a variety of different binding assays, provided they are performed under conditions where the dissociation of the steroid from the receptor is slow.
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