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  • Title: Specific enzyme-linked immunoassay for rotavirus serotypes 1 and 3.
    Author: Shaw RD, Stoner-Ma DL, Estes MK, Greenberg HB.
    Journal: J Clin Microbiol; 1985 Aug; 22(2):286-91. PubMed ID: 2993354.
    Abstract:
    We prepared monoclonal antibodies against two serotypically distinct rotavirus strains: Wa, a serotype 1 virus of human origin, and rhesus rotavirus, a simian serotype 3 virus. Monoclonal antibodies which react specifically with VP7 of each serotype were identified by hemagglutination inhibition tests, plaque reduction neutralization studies, and solid-phase immunoassays which used wild-type and reassortant strains of rotavirus. An enzyme-linked immunoassay was designed which utilizes two of these antibodies to correctly identify serotype 1 and serotype 3 viruses.
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