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  • Title: Monoclonal antibodies recognizing early and late antigens of human cytomegalovirus: heterogeneity of polypeptides recognized between virus isolates.
    Author: Rodgers BC, Mundin J, Sissons JG.
    Journal: J Gen Virol; 1985 Sep; 66 ( Pt 9)():2045-9. PubMed ID: 2993489.
    Abstract:
    The characteristics of four human cytomegalovirus (HCMV)-specific monoclonal antibodies as assessed by ELISA, immunofluorescence, immunoprecipitation and Western blotting are described. Two antibodies recognized a 67K late polypeptide of HCMV, one recognized 43K and 79K polypeptides present early and late in HCMV-infected cells, and the fourth identified a 72K early nuclear protein of HCMV. The antibodies recognized these antigens in all HCMV isolates tested by immunofluorescence and ELISA, but demonstrated inter-isolate variations in polypeptides recognized by Western blotting.
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