These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: A temperature-sensitive mutation constructed by "linker insertion" mutagenesis.
    Author: Colicelli J, Lobel LI, Goff SP.
    Journal: Mol Gen Genet; 1985; 199(3):537-9. PubMed ID: 2993803.
    Abstract:
    The in vitro mutagenesis of cloned DNAs allows the formation of virtually any specific mutation, but no method has been found which might routinely lead to the important phenotype of temperature sensitivity. We have studied three linker insertion mutations in the envelope gene of Moloney murine leukemia virus (M-MuLV), and found that one was exquisitely temperature-sensitive for plaque formation. We suggest that the construction of short insertion mutations may be a fruitful approach for the generation of temperature-sensitive phenotypes in cloned genes.
    [Abstract] [Full Text] [Related] [New Search]