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  • Title: An electrochemical biosensor for the detection of Pb2+ based on G-quadruplex DNA and gold nanoparticles.
    Author: Xu S, Chen X, Peng G, Jiang L, Huang H.
    Journal: Anal Bioanal Chem; 2018 Sep; 410(23):5879-5887. PubMed ID: 29959487.
    Abstract:
    We present a novel simple strategy for the detection of Pb2+ based on G-quadruplex DNA and gold nanoparticles. First, gold nanoparticles were chemically adsorbed onto the surface of a thiol-modified gold electrode. Subsequently, the substrate DNA1 was adsorbed onto the surfaces of the gold nanoparticles via thiol-gold bonds, so that the complementary guanine-rich DNA2 could be hybridized to the gold electrode in sequence. [Ru(NH3)6]3+ (RuHex), which can be electrostatically adsorbed onto the anionic phosphate of DNA, served as an electrochemical probe. The presence of Pb2+ can induce DNA2 to form a stable G-quadruplex and fall off the gold electrode. The amount of RuHex remaining on the electrode surface was determined by electrochemical chronocoulometry (CC). The prepared biosensor showed high sensitivity for Pb2+ with a linear range with respect to ln(cPb2+) from 0.01 to 200 nM and a low detection limit of 0.0042 nM under optimal conditions. Because of the high selectivity of the Pb2+-specific DNA2, the designed biosensor also showed low false-positive signal rates with other metal ions in real-world examples. Therefore, this strategy has the potential for practical application in environmental monitoring. Graphical abstract ᅟ.
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