These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Fluorometric aptamer-based determination of ochratoxin A based on the use of graphene oxide and RNase H-aided amplification.
    Author: Ma C, Wu K, Zhao H, Liu H, Wang K, Xia K.
    Journal: Mikrochim Acta; 2018 Jun 30; 185(7):347. PubMed ID: 29961128.
    Abstract:
    The authors describe a fluorometric assay for ochratoxin A (OTA) that is based on the use of graphene oxide and RNase H-aided amplification. On addition of OTA, cAPT is replaced from the APT/cAPT hybridization complex and then hybridizes with RNA labeled with a fluorophore at the 5'-end. Eventually, the fluorophore is released by RNase H cleavage. As the concentration of OTA increases, more cAPTs are displaced, this leading to fluorescence enhancement (best measured at excitation/emission wavelengths of 495/515 nm). This RNase H-assisted cycle response results in strong signal amplification. The limit of detection, calculated on the basis of a signal to noise ratio of 3, is 0.08 ng·mL-1. Response is linear in the 0.08-200 ng·mL-1 OTA concentration range. The method is highly selective for OTA over ochratoxin B and aflatoxin B1. It was applied to the determination of OTA in red wine samples spiked at levels of 1, 7, and 50 ng·mL-1, and the recoveries ranged from 90.9 to 112%. Graphical abstract Schematic of a novel fluorometric aptasensor for ochratoxin A based on the use of graphene oxide and RNase H-aided amplification.
    [Abstract] [Full Text] [Related] [New Search]