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  • Title: Immunoperoxidase staining for the detection of herpes simplex virus antigen in cervicovaginal smears.
    Author: Iwa N, Katayama Y, Ito K, Nishiura H, Ueda S, Kato S.
    Journal: Acta Cytol; 1985; 29(5):705-7. PubMed ID: 2996270.
    Abstract:
    Destained cervicovaginal smears from eight patients with herpes simplex virus (HSV) infections were stained by means of the peroxidase-antiperoxidase (PAP) technique to demonstrate the presence of the HSV type 2 (HSV-2) antigen. Positive results were obtained in six of the eight cases, with intense staining for the HSV-2-specific antigen throughout the cytoplasm and nuclei of cells having a ground-glass nuclear appearance as well as in multinucleated giant cells. Virus isolation was successfully performed for the HSV-2-positive case that also had a histologically confirmed squamous-cell carcinoma of the cervix. The combined use of cytology and the PAP staining technique was of great value in the demonstration of cervical HSV infections.
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