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  • Title: Preparation and reconstitution of a phospholipid deficient cytochrome b6-f complex from spinach chloroplasts.
    Author: Doyle MF, Yu CA.
    Journal: Biochem Biophys Res Commun; 1985 Sep 16; 131(2):700-6. PubMed ID: 2996523.
    Abstract:
    A simple, rapid procedure suitable for large scale preparation of a lipid deficient cytochrome b6-f complex from spinach chloroplasts has been developed. The procedure involves solubilization with a mixture of sodium cholate and octylglucoside, ammonium sulfate fractionation and calcium phosphate column chromatography. The purified complex contains, in nanomoles per milligram protein, 20.6 cytochrome b, 10.8 cytochrome f and 54 phospholipids. The purified complex has little plastoquinol-cytochrome c reductase activity in the absence of added lipid. Full reductase activity was reconstituted by the addition of plastoquinone prior to the addition of lipid.
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