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  • Title: Characterization of a novel hydroxynitrile lyase from Nandina domestica Thunb.
    Author: Isobe K, Kitagawa A, Kanamori K, Kashiwagi N, Matsui D, Yamaguchi T, Fuhshuku KI, Semba H, Asano Y.
    Journal: Biosci Biotechnol Biochem; 2018 Oct; 82(10):1760-1769. PubMed ID: 29975178.
    Abstract:
    The leaves of Nandina domestica Thunb. exhibited high hydroxynitrile lyase (HNL) activity in (R)-mandelonitrile synthesis. The specific activity of young leaves was significantly higher than that of mature leaves. We isolated two HNLs with molecular mass of 24.9 kDa (NdHNL-S) and 28.0 kDa (NdHNL-L) from the young leaves. Both NdHNLs were composed of two identical subunits, without FAD and carbohydrates. We purified NdHNL-L and revealed its enzymatic properties. The whole deduced amino acid sequence of NdHNL-L was not homologous to any other HNLs, and the specific activity for mandelonitrile synthesis by NdHNL-L was higher than that by other plant HNLs. The enzyme catalyzed enantioselective synthesis of (R)-cyanohydrins, exhibited high activity at pH 4.0, and high stability in the pH range of 3.5-8.0 and below 55°C. Thus, NdHNL-L is a novel HNL with novel amino acid sequence and has a potential for the efficient production of (R)-cyanohydrins.
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