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  • Title: Effect of proteolysis on the electron spin resonance spectra of maleimide spin labeled erythrocyte membrane.
    Author: Bartosz G, Gaczyńska M.
    Journal: Biochim Biophys Acta; 1985 Dec 05; 821(2):175-8. PubMed ID: 2998467.
    Abstract:
    The ratio of low-field amplitudes of weakly and strongly immobilized signals of ESR spectra of a maleimide spin label bound to erythrocyte membranes (hw/hs) increases progressively during incubation at 37 degrees C. This increase is due to the 'self-digestion' of membrane proteins by endogenous proteinases and is attenuated by proteinase inhibitors. Digestion of membranes with chymotrypsin also increases the hw/hs ratio. These results suggest a need for a careful interpretation of data from spin-labeled membrane proteins, especially in experiments involving prolonged incubations of membrane preparations when the proteolytic effects may be significant.
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