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  • Title: Studies on the eye lens in poikilothermal animals. II. Stimulation of anaerobic glycolysis in rainbow trout lenses incubated with Ca2+-free medium.
    Author: Hikida M, Iwata S.
    Journal: Exp Eye Res; 1985 Aug; 41(2):179-82. PubMed ID: 2998851.
    Abstract:
    The effects of Ca2+-free medium on cation levels and lactic acid production of rainbow trout lens incubated at various temperatures were examined in order to study passive cation transport system. Na+-K+ ratio (Na+/K+) increased when the lens was treated with Ca2+-free medium, but the effect of Ca2+-free medium was lowest between 15 and 20 degrees C which was the optimum water temperature for rainbow trout. Lactic acid production was stimulated between 15 and 20 degrees C in Ca2+-free medium. It was also stimulated at 37 degrees C in rat lenses incubated with Ca2+-free medium. However, the production was suppressed in ouabain-treated rainbow trout lenses. The suppression of Na+/K+ increase between 15 and 20 degrees C disappeared when the lens was treated with both Ca2+-free medium and ouabain. These results suggest that Na,K-ATPase activity and glycolysis were stimulated to prevent the increase of Na+/K+ in Ca2+-free medium. In addition, these results indicate that the optimum temperature was between 10 and 20 degrees C for the rainbow trout lens incubation in vitro, which is also the optimum water temperature in vivo.
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